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Addgene
Showing: 1981 - 2000 of 10430 results

  11. pCK301

    Plasmid
    #87767
    Purpose
    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Insert
    PrhaBAD-sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli
    Availability
    Academic Institutions and Nonprofits only
    87767-plasmid-map-sequence-id-177279

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  12. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  14. pHES839

    Plasmid
    #87941
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Availability
    Academic Institutions and Nonprofits only
    87941-plasmid-map-sequence-id-180360

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Showing: 1981 - 2000 of 10430 results