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Plasmid#89238PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusionDepositorInsertCas2/3
UseTagsStrep II-MBPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+7U
Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2-WPRE
Plasmid#218871PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterGFAPAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE
Plasmid#218873PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVTagsExpressionMammalianMutationS99A F102G R168PPromoterGFAPAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2(no bind)-WPRE
Plasmid#218876PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVTagsExpressionMammalianMutationS99A F102G R168PPromoterSynapsinAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2-WPRE
Plasmid#218880PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-flex-iGABASnFR2n-WPRE
Plasmid#218878PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp
Plasmid#65024PurposeTpR mini-Tn7 vector, mobilizableDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-AAV-CAG-flex-iGABASnFR2n-WPRE
Plasmid#218881PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-mcBEST
Plasmid#209416PurposePlasmid for multiplexed cytosine base editing in streptomycetesDepositorInsertsCodon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterbase editing cassette: PtipA ; sgRNAs: PkasO* an…Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only