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Plasmid#182310PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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XLone-GFP
Plasmid#96930PurposeTunable and temporal expression control of GFPDepositorInsertGreen Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2-EM7neoPheS
Plasmid#112923PurposepiggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)DepositorInsertneoPheS
UsePiggybac transposon gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-mCherry
Plasmid#170519PurposePlasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-mCherry
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-empty-pgk-hph
Plasmid#48753PurposePiggyBac backbone, CAG promoter, Hygromycin selectionDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E
Plasmid#212657PurposepiggyBac vector to express an MLV Gag-EIF4E fusion proteinDepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4EExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Venus
Plasmid#170518PurposePlasmid containing piggyBac inverted terminal repeats (ITRs) sequences flanking CAG and VenusDepositorInsertCAG-Venus
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-U6insert-EF1puro
Plasmid#104537PurposeA piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …TagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEP179_pX330K
Plasmid#137882PurposeEntry vector for multi-gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-U6
Plasmid#120358PurposepiggyBac transposon vector for sgRNA expression.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PBMBP-eGFP
Plasmid#40976DepositorInsertMBP promoter fragment (Mbp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG-empty-pgk-hph
Plasmid#48754PurposePiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tagDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-sHRPaNRX3beta
Plasmid#79910PurposepiggyBac transposon vector, sHRPaNRX3betaDepositorInsertsHRPaNRX3beta
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
S6_BstBI_SV40_SwaI
Plasmid#128470PurposeSV40 promoterDepositorInsertSV40 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
S13_EcoRV_UBC_AscI
Plasmid#128477PurposeUBC promoterDepositorInsertUBC promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S12_EcoRV_PGK_AscI
Plasmid#128476PurposePGK promoterDepositorInsertPGK promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E-TM
Plasmid#212658PurposepiggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)DepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4E (W56L, W102L, W166L)ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag∆MA
Plasmid#212654PurposepiggyBac vector to express MLV Gag with truncated MA domainDepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsExpressionMammalianMutationDeletion of MA domain (amino acids 2-215)PromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only