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PB-U6

Plasmid

#120358

Purpose

piggyBac transposon vector for sgRNA expression.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

Promoter

TRE3G-Bi-directional

Availability

Academic Institutions and Nonprofits only

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Dox-SOScat

Plasmid

#207329

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

son of sevenless homolog 2 variant, catalytic domain

Use

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 593 to 1078

Promoter

Tet Response Element

Availability

Academic Institutions and Nonprofits only

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pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

EF1-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1 promoter

Availability

Academic Institutions and Nonprofits only

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pMK459 (dTAG-BromoTag-mAID-EGFP)

Plasmid

#214387

Purpose

dTAG-BromoTag-mAID-EGFP reporter (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

dTAG-BromoTag-mAID-EGFP reporter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Tfe3-pgk-hph

Plasmid

#48755

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3 (Tcfe3 Mouse)

Use

Piggybac

Tags

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1

Plasmid

#79912

Purpose

piggyBac transposon vector, Cerulean-2A-sHRPbNLG1

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Cerulean-2A-sHRPbNLG1

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

Plasmid

#79913

Purpose

piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Zeocin-t2A-sHRPbNRX3beta

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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S7_SwaI_Zeo_PacI

Plasmid

#128471

Purpose

Zeo Resistance gene

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Zeocin Resistance gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-EF1a-TetOn3G

Plasmid

#104543

Purpose

PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

Tet-On 3G

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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1137=Hsp70Bb-Cas9[dsRed]

Plasmid

#149424

Purpose

The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

Cas9

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Dmel Hsp70Bb

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

Plasmid

#162804

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI_IRES_Blasticidin

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Tfe3::ERT2-pgk-hph

Plasmid

#48756

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3::ERT2 (Tcfe3 Mouse)

Use

Piggybac

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only
PB-Mycn

Plasmid

#60667

Purpose

PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Mycn (Mycn Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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