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Showing: 181 - 200 of 235 results

  1. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  2. pM2s2AsR_TS-E10

    Plasmid
    #137944
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    mRFP1
    mWasabi
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 and pTlpA-C7
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137944-plasmid-map-sequence-id-331371

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  3. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  4. pTd-4-15-13-10

    Plasmid
    #63835
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  6. pTd-4-15-13-1

    Plasmid
    #63826
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    63826-plasmid-map-sequence-id-106559

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  7. pTd-4-15-13-2

    Plasmid
    #63827
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pTd-4-15-13-3

    Plasmid
    #63828
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pTd-4-15-13-4

    Plasmid
    #63829
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pTd-4-15-13-5

    Plasmid
    #63830
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pTd-4-15-13-6

    Plasmid
    #63831
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pTd-4-15-13-7

    Plasmid
    #63832
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pTd-4-15-13-8

    Plasmid
    #63833
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pTd-4-15-13-9

    Plasmid
    #63834
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pTd-4-15-13-11

    Plasmid
    #63836
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 235 results