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Plasmid#118694PurposeExpress sgZFP64e2 resistant cDNADepositorInsertZFP64 (ZFP64 Human)
UseLentiviralTagsExpressionMutationsgZFP64e2 targeting site was synonymous mutatedPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_ZFP64_Delta_TAD
Plasmid#118698PurposeExpress ZFP64 without transcription activation domain(TAD)DepositorInsertZFP64_Delta_TAD (ZFP64 Human)
UseLentiviralTags3XFLAG was fused to the N terminal of ZFP64 cDNAExpressionMutationDelete amino acid from 453 to 681PromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Neo_ZFP64_N_3XFLAG
Plasmid#118700PurposeExpress 3xflag tagged ZFP64DepositorInsertZFP64 (ZFP64 Human)
UseLentiviralTags3XFLAG was fused to the N terminal of ZFP64 cDNAExpressionMutationPromoterEF1AAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LentiV_Neo_LKB1_CR
Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-jAspSnFR3
Plasmid#203488PurposejAspSnFR3 in lentiviral expression vectorDepositorInsertjAspSnFR3
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast
Plasmid#52962PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.DepositorHas ServiceLentiviral PrepInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-mCherry
Plasmid#99154PurposeLentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.DepositorInsertsCas9
mCherry
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Blast
Plasmid#199622PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-zeo
Plasmid#160091PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-V5-KDM3A
Plasmid#82331Purposelentiviral expression of KDM3ADepositorInsertKDM3A (KDM3A Human)
UseLentiviralTagsV5ExpressionMammalianMutationtwo silent mutations to make it siRNA resistantPromoterAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CR029
Plasmid#234041Purposeexpresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmidDepositorInsertevoCDA1-Cas9(D10A)-P2A-blast
UseLentiviralTagsblastExpressionMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CR033
Plasmid#234042Purposeexpresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmidDepositorInsertTadA8e(V106W)-Cas9(D10A)-P2A-blast
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CR102
Plasmid#234043Purposeexpresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmidDepositorInsertTadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only