Addgene

We narrowed to 2,412 results for: golden gate

Showing: 181 - 200 of 2412 results

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pMEG_BB3_YL68N_AD

Plasmid

#117831

Purpose

GoldenMOCS backbone for 3 TU in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDM098

Plasmid

#216818

Purpose

Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Insert

mStayGold

Use

Tags

FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGGDestSC+ATG

Plasmid

#49323

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGW_SM_BamHI

Plasmid

#49315

Purpose

Subcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insert

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p3E_GGWDest-

Plasmid

#49316

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest+

Plasmid

#49319

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_GGWDest+

Plasmid

#49321

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_XcmI-LacZ

Plasmid

#49296

Purpose

Golden Gate entry vector at position 1, no 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGW_SM_BglII

Plasmid

#49314

Purpose

Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E_GGWDest+

Plasmid

#49317

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_GGWDest-

Plasmid

#49320

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_Linker

Plasmid

#49281

Purpose

Golden Gate entry vector at position 1, small linker insert, no 5' ATG

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1A

Plasmid

#102701

Purpose

Mobius Assembly Level 1 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Level 2A

Plasmid

#102705

Purpose

Mobius Assembly Level 2 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:sfGFP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Level 1Δ

Plasmid

#102704

Purpose

Mobius Assembly Level 1 Acceptor Vector Δ

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge