pGGW_SM_BglII
(Plasmid
#49314)
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Purpose(Empty Backbone) Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR
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Backbone manufacturerInvitrogen
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGW_SM_BglII was a gift from Joachim Wittbrodt (Addgene plasmid # 49314 ; http://n2t.net/addgene:49314 ; RRID:Addgene_49314) -
For your References section:
Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. Kirchmaier S, Lust K, Wittbrodt J. PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117. 10.1371/journal.pone.0076117 PubMed 24116091