Addgene

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pJH375

Plasmid

#86841

Purpose

mamalian expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMK11-02

Plasmid

#99606

Purpose

Plasmid containing parBp(mut)-mKate2 fusion

Depositor

Morten Kjos, Jan-Willem Veening

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

parBp(mut)-mKate2

Use

Synthetic Biology

Tags

mKate2

Expression

Mutation

internal parS-site mutated

Promoter

PczcD

Available since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCSW24

Plasmid

#86837

Purpose

bacterial expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-SrtAstrep (pKS78)

Plasmid

#50029

Purpose

Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

Plasmid

#50033

Purpose

Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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A9QH69

Plasmid

#163216

Purpose

Inducible expression of UniProt identifier A9QH69

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A9QH69

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDAC439

Plasmid

#195237

Purpose

Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-J6-dCas9-24XGP41-J9

Plasmid

#202006

Purpose

MoClo level 0 dCas9-24XGP41 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo cloning vector

Tags

GS linkers

Expression

Mutation

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pTdTomato-L5

Plasmid

#140994

Purpose

tdTomato, StrepR, L5int, attP to create RFP mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

hsp60

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL0_12 [pAMβ1 ORI]

Plasmid

#198926

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMC-J6-dCas9-10XGP41-J9

Plasmid

#202005

Purpose

MoClo level 0 dCas9-10XGP41 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Insert

dCas9-10XGP41

Use

Synthetic Biology; Moclo cloning vector

Tags

GS linkers

Expression

Mutation

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDAC569

Plasmid

#195241

Purpose

Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Tags

Expression

Mutation

Promoter

P23

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-nCas9n-A71

Plasmid

#125644

Purpose

micro-injection of poly-adenylated nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

Promoter

T3

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPM_Pveg-sfgfp

Plasmid

#121503

Purpose

Integration vector that expresses gfp in S. mutans

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

Superfolder GFP

Use

S. mutans integration vector, integrates at the m…

Tags

Expression

Mutation

Promoter

Pveg

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX165-HiFi Cas9

Plasmid

#140563

Purpose

Expresses HiFi Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Use

Tags

Expression

Mutation

R691A

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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