Addgene

We narrowed to 1,251 results for: G3

Showing: 181 - 200 of 1251 results

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pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179027

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Tags

Expression

Mutation

Promoter

3XP3

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pKF295

Plasmid

#72871

Purpose

expression of improved FLP recombinase

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

FLP recombinase (flp Budding Yeast)

Use

Tags

Expression

Insect

Mutation

P2->S, added NLS

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Tags

Expression

Mutation

Promoter

vasa

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCFD4d

Plasmid

#83954

Purpose

Modified from pCFD4, with the vermillion marker and the attB site removed

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

dU6-1; dU6-3

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCN-MET3-GFP

Plasmid

#45339

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

MET3

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCN-HHT2-GFP

Plasmid

#45322

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

HHT2

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCN-EGD2-GFP

Plasmid

#45318

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

EGD2

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCN-ACO2-GFP

Plasmid

#45330

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

ACO2

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRVV661

Plasmid

#108282

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCN-LYS21-GFP

Plasmid

#45335

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

LYS21

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-TetR-cryaa-YFP

Plasmid

#73202

Purpose

BAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRB37

Plasmid

#72869

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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