These results may also be relevant.
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pRATIO2151
Plasmid#141300PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneUseTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK028
Plasmid#171300PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert1xQUAS UAS (for fusion)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_00057131
Plasmid#121300PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_00057131
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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gnu-GFP 9A
Plasmid#113006PurposeP element vector for transposon insertion of gnu-GFP 9A into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutation9 CycB/CDK1 sites mutated to APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu-GFP WT
Plasmid#113005PurposeP element vector for transposon insertion of gnu-GFP WT into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu 9A
Plasmid#113000PurposeFor protein expression and purification of full-length Drosophila gnu 9ADepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutation9 CycB/CDK1 sites mutated to APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-ZC3H12B
Plasmid#221300PurposeExpression of ZC3H12B (pLxIS) in mammalian cells by retroviral transductionDepositorInsertZC3H12B (pLxIS)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP4-Cd4-bio-His
Plasmid#126843PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP4 (PKH_041300 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2FE-ABE8e-SpRY
Plasmid#213008PurposeA lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning siteDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorInsertfull length me31B (me31B Fly)
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Amber-free HIV-1 deltaRT
Plasmid#213007PurposeAn replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.DepositorInsertsHIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_ABE8e-SpRY-P2A-BFP_HygroR
Plasmid#213009PurposeA lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFPDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral N
Plasmid#113009PurposeFor bacterial expression of MBP fusion of N terminal region of Drosophila TralDepositorInsertN terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 1-355PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C
Plasmid#113008PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila TralDepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 356-652PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C 15A
Plasmid#113007PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)DepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationPNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FYB-2/2
Plasmid#91300PurposeProtein expression and purification of human SH3 domain construct FYB-2/2DepositorInsertFYB-2/2 (FYB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Target-AID (pRZ762)
Plasmid#131300PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.DepositorInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only
