-
Plasmid#74705PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) TFRC
Plasmid#74709PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
Plasmid#83491Purposecre-dependent DN-AMPK under EF1a promoterDepositorInsertDN-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationK45RPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB1609_pRR-EGFP
Plasmid#65852PurposeEGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit EGFP coding sequence
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterSV40Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2
Plasmid#60388PurposeExpression of yeast beta tubulin (TUB2) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKP1627
Plasmid#167648PurposeExpress FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C, delta12-16 (TonB box)
UseTagsExpressionBacterialMutationfepA with G54C, delta12-16 (TonB box) mutations (…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1626
Plasmid#167646PurposeExpress FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C, delta12-16 (TonB box)
UseTagsExpressionBacterialMutationfepA with D34C, delta12-16 (TonB box) mutations. …PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSPIKE-P
Plasmid#101172Purposechimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomicsDepositorInsertV4 16S rRNA chimeric spike
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1610_pRR-Puro
Plasmid#65853PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit puromycin N-acetyltransferase coding sequence
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterSV40Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1
Plasmid#60385PurposeExpression of yeast alpha tubulin (TUB1) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2-Cterminal6xHis
Plasmid#60386PurposeExpression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoterDepositorInsertTUB2
UseTags6xHISExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB1-Cterminal6xHis
Plasmid#60387PurposeExpression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTags6xHISExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 (1-428)
Plasmid#60395PurposeExpression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationdeleted 429 - endPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-actin E6 E7
Plasmid#13712DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pED7x26
Plasmid#134813PurposeStringent phage propagation reporter containing pIII-16-29-34-TAGADepositorInsertpIII 16-TAGA 29-TAGA 34-TAGA
UseSynthetic BiologyTagsExpressionBacterialMutation16-TAGA 29-TAGA 34-TAGAPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGC461
Plasmid#71363Purposedistal tip cell expression of DAF-16 in C. elegansDepositorInsertdaf-16 (daf-16 Nematode)
UseTagsGFP(65C)ExpressionWormMutationPromoterBlelloch et al., 1999; Mathies et al., 2003Available sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -