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Plasmid#188327PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mCherry::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS011
Plasmid#188334PurposePCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mTagBFP2::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
UseTagsExpressionWormMutationPromotersdhb-1Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNMSB99
Plasmid#199308PurposeMosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertmyo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promotermyo-3Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM1.151
Plasmid#21386DepositorInsertmCherry::histone H2B in pDONR201
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEY51
Plasmid#191054Purposemec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmec-3 (mec-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK2014
Plasmid#171931PurposeTo express sygl-1 fused to 24 copies of MS2 loops.DepositorInsertPsygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
UseTagsN-terminal Tag 24X MS2 loopsExpressionWormMutationPromoter-Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN267
Plasmid#86723PurposeTissue-specific FLP expression plasmid for C. elegansDepositorInsertPrgef-1::FLP D5
UseTagsExpressionWormMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEY11
Plasmid#191017PurposeFluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertUltrapanneuronal
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY26
Plasmid#191031Purposecho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.3-2.6k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY39
Plasmid#191043Purposeacr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(2.1k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TC872
Plasmid#164895PurposeTRE::nCre, inducible nCre expression when co-express with rtTADepositorInsertnCre
UseTagsExpressionWormMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY52
Plasmid#191055Purposeodr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-1 (odr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TC513
Plasmid#122616PurposePunc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherryDepositorInsertSV40 NLS::rtetR::Zip(+)::SL2::mCherry
UseTagsExpressionWormMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAH66 - [BAGp | gfp | tbb-2 UTR]
Plasmid#200340Purposegfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001536, flp-17Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]
Plasmid#200339PurposemScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001536, flp-17Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TC476
Plasmid#122629PurposePunc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
UseTagsExpressionWormMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only