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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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hCEP120-Myc

Plasmid

#50381

Purpose

Tet-On inducible expression of Myc-tagged hCEP120

Depositor

Tang Tang

Article

Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

Insert

Human CEP120 (CEP120 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

This hCEP120 contains 4 SNPs (rs6876883, rs659544…

Promoter

CMV-2xTetO

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆PABC

Plasmid

#81064

Purpose

Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)

Use

Cloning

Tags

Expression

Mutation

Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305A-3xFLAG

Plasmid

#69839

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305A

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-Cenexin-S796A

Plasmid

#186434

Purpose

Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

Insert

Cenexin (ODF2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 796 to Alanine

Promoter

CMV IE94

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep170 (Nigg PID200)

Plasmid

#41150

Depositor

Erich Nigg

Article

Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

Insert

Cep170 (CEP170 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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