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Showing: 1 - 20 of 34 results

  8. mCherry-FDD

    Plasmid
    #80629
    Purpose
    Fluorescent destablizing domain for regulating protein stability and fluorescence
    Depositor
    Thomas Wandless
    Article
    Navarro et al ACS Chem Biol. 2016 Jun 6.
    Insert
    UnaG
    Use
    Retroviral
    Tags
    HA-mcherry-linker
    Expression
    Mutation
    mutations A36V, R136G
    Promoter
    LTR
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    80629-plasmid-map-sequence-id-156876

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  13. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  14. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pBMN ltDHFR(DD)-YFP

    Plasmid
    #47076
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Expression
    Mutation
    amino acids mutations: R12H, N23S, G67S, V78A, E1…
    Promoter
    LTR
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47076-plasmid-map-sequence-id-67102

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Showing: 1 - 20 of 34 results