Addgene

Showing: 1 - 20 of 334 results

Results per page:

Sort results by:

pUC18

Plasmid

#50004

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-iSpinach

Plasmid

#186053

Purpose

template for co-transcriptional RNA folding assays

Depositor

Markus Wahl

Article

Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

Insert

T7A1-rrnG-iSpinach

Use

Tags

no

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-GFP

Plasmid

#220495

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18-LucFF

Plasmid

#220496

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Rp51a_No_BP-pUC18

Plasmid

#100992

Purpose

Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

TACTAAC->GTTAGTG BP mutataion

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18_BCR_GUSB_ABL1_e14a3

Plasmid

#190885

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e14a3 BCR::ABL1 fusion transcript
ABL1 cDNA (ABL1 Human)
GUSB cDNA (GUSB Human)
BCR (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18_BCR_GUSB_e19a2

Plasmid

#190884

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e19a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18_BCR_GUSB_e6a2

Plasmid

#190882

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e6a2 cDNA
GUSB cDNA transcript (GUSB )
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18_BCR_GUSB_e8a2

Plasmid

#190883

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e8a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-H1 RNAi

Plasmid

#87355

Purpose

RNAi expression vector based on H1 promoter and pUC18 vector backbone

Depositor

Saroj Mathupala

Article

Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

Insert

H1 RNA Promoter from U87MG glioma cells

Use

RNAi; Shrna expression vector

Tags

Expression

Mammalian

Mutation

Promoter

H1 RNA

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18T-mini-Tn7T-Gm

Plasmid

#63121

Purpose

mini-Tn7 vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18-mini-Tn7T-Gm-GW

Plasmid

#64961

Purpose

mini-Tn7 Gateway destination vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18R6K-mini-Tn7T-Gm

Plasmid

#65022

Purpose

GmR mini-Tn7 vector, R6K replicon

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18R6K-mini-Tn7T

Plasmid

#64958

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-mini-Tn7

Plasmid

#64953

Purpose

mini-Tn7 base vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge