Rp51a_No_BP-pUC18
(Plasmid
#100992)
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PurposeContains model Rp51a with a TACTAAC->GTTAGTG BP mutataion
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRP51A
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SpeciesS. cerevisiae (budding yeast)
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MutationTACTAAC->GTTAGTG BP mutataion
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Entrez GeneRPS17A (a.k.a. YML024W, RP51A, RPL51A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rp51a_No_BP-pUC18 was a gift from Aaron Hoskins (Addgene plasmid # 100992 ; http://n2t.net/addgene:100992 ; RRID:Addgene_100992) -
For your References section:
Dynamics and consequences of spliceosome E complex formation. Larson JD, Hoskins AA. Elife. 2017 Aug 22;6. pii: 27592. doi: 10.7554/eLife.27592. 10.7554/eLife.27592 PubMed 28829039
