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PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA
Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_OTX2_P2A_Hygro_Barcode
Plasmid#120471PurposeBarcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTX2-donor
Plasmid#176358PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertOTX2 (OTX2 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTX2.1.0-gDNA
Plasmid#176376PurposeExpresses spCas9 and gRNA sequence targeting OTX2DepositorInsertOTX2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA1
Plasmid#73979PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA2
Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA2
Plasmid#73980PurposeOtx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorInsertOtx2(isoform b)-Ires-GFP (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B108-Otx2-IRES-Cre
Plasmid#73993PurposeB108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.DepositorInsertB108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Otx2-IRES-EGFP
Plasmid#153947PurposepiggyBac transposon vector with CAG promoter expressing Otx2 and EGFPDepositorInsertOtx2 (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)
Plasmid#171102PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.DepositorInserthSpCas9 (Otx2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CLYBL hOPM
Plasmid#112499PurposeHomologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locusDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS hOPM
Plasmid#112498PurposeHomologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.DepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMin-DHS-4D-GFP
Plasmid#159653PurposeReporter plasmid for the DHS-4D Otx2 enhancer element.DepositorInsertDHS-4D
UseTagsExpressionMammalianMutationPromoterTATAAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only
