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Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusTagsExpressionMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_vulnificus
Plasmid#224405PurposeFor expression of the type I CBASS system encoded by Vibrio vulnificusDepositorInsertEndonuclease_Phospholipase_VvCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTDSP2_NIF
Plasmid#109831PurposeProtein expression and purification of CTDSP2_NIFDepositorInsertCTDSP2_NIF (CTDSP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
nifF in pET21b
Plasmid#124520PurposeAzotobacter vinelandii flavodoxin II of NitrogenaseDepositorInsertFlavodoxin II (nifF Azotobacter vinelandii)
UseTagsNoneExpressionBacterialMutationPromoterT7 / lacAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-SNIFP-N1
Plasmid#197189PurposeExpresses the fusion protein of CytERM-SNIFP in mammalian cellsDepositorInsertCytERM-SNIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCiPS-NIFV
Plasmid#110807PurposeNon-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cellsDepositorInsertPol
UseTagsExpressionMammalianMutation2 mutations in the DDE catalyic core motif of the…PromoterCMVAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX_Snifit
Plasmid#119043PurposePlasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)DepositorInsertMTX_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-mito
Plasmid#113916PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-nucl
Plasmid#113915PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-nucl
Plasmid#113918PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SNIFP
Plasmid#197210PurposeExpresses the protein of SNIFP in mammalian cellsDepositorInsertSNIFP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NAD-Snifit
Plasmid#113658PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentrationDepositorInsertNAD-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CoA-Snifit(G41)
Plasmid#197945PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only