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Plasmid#206039PurposeHaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTagDepositorInsertTalin R3-IVVI (Tln1 Mouse)
UseTags10 x His-AviTag and Halo-TagExpressionBacterialMutationThreonine 809 to Isoleucine, Threonine 833 to Val…PromoterT7Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorInsertBiotin (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC
Plasmid#221349PurposeMammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.DepositorInsertAnti-Biotin [IPI-M33] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
Plasmid#221350PurposeMammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.DepositorInsertAnti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCherryExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)
Plasmid#29725DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and GFPExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)
Plasmid#29723DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)
Plasmid#29724DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCitrineExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneUseTagsBiotin-H6-TEV recognition and FlAsHExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTSA-38
Plasmid#17328DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pD451-pA-APEX2
Plasmid#157863PurposeBacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labelingDepositorInsertpA-APEX2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur/GDI1-splitTurboID
Plasmid#198219PurposeTransposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labelingDepositorInsertGID1
UseTransposonTags5’ part of TurboID, up to E256ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDW464
Plasmid#8845DepositorTypeEmpty backboneUseTagsbiotin acceptor peptideExpressionInsectMutationPromoterAvailable sinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDW465
Plasmid#8846DepositorTypeEmpty backboneUseTagsbiotin acceptor peptideExpressionInsectMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDW446
Plasmid#8844DepositorTypeEmpty backboneUseTagsbiotin acceptor peptideExpressionInsectMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDW445
Plasmid#8843DepositorTypeEmpty backboneUseTagsbiotin acceptor peptideExpressionInsectMutationPromoterAvailable sinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/mCardinal-TurboID-GAI
Plasmid#198218PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labelingDepositorInsertmCardinal
UseTransposonTags3’ part of TurboID, starting with G257 and amino …ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/PAG1-TurboID-GAI
Plasmid#198217PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labelingDepositorInsertPAG1 (PAG1 Human)
UseTransposonTags3’ part of TurboID, starting with G257 and amino …ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBABE-puro
Plasmid#80901PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorTypeEmpty backboneUseRetroviralTagsBirA(R118G) and mycExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only