Skip to main content
Addgene
Showing: 1 - 40 of 1215 results

  2. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

    Enlarge

  8. MCP-EGFP-PiggyBac

    Plasmid
    #190003
    Purpose
    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.
    Depositor
    Tineke Lenstra
    Article
    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi
    Insert
    MCP-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    190003-plasmid-map-sequence-id-375799

    Enlarge

  11. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

    Enlarge

  13. piggyBAC-BRD4-Flag

    Plasmid
    #214432
    Purpose
    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.
    Depositor
    Shaun Cowley
    Insert
    Brd4 bromodomain containing 4 (Brd4 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    214432-plasmid-map-sequence-id-437159

    Enlarge

  32. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid
    #211821
    Purpose
    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment
    Depositor
    Erik Sontheimer
    Article
    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.
    Insert
    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken β-actin promoter, U6 promoter and hPGK pr…
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211821-plasmid-map-sequence-id-418440

    Enlarge

Showing: 1 - 40 of 1215 results