Addgene

Showing: 1801 - 1820 of 7146 results

Results per page:

Sort results by:

pVG45

Plasmid

#164703

Purpose

yEVenus under the control of CUP1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

CUP1pr - yEVenus - CLN2 PEST - ADH1t

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAS1052

Plasmid

#158598

Purpose

VioA, VioB

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

VioA
VioB

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-RPL13A-FKBPx2

Plasmid

#104473

Purpose

Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

RPL13Ap (RPL13A Budding Yeast)

Use

Tags

FKBPx2-HA

Expression

Yeast

Mutation

includes the last 354 bp of RPL13A only

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp204-PADH1-AFB2

Plasmid

#99531

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXP222

Plasmid

#26833

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER

Plasmid

#52687

Purpose

Same as 41025, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-GFP+LINKER

Use

Promoter replacement

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-APL2-iGFPx6

Plasmid

#105251

Purpose

Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

APL2 portion (APL2 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-3GFP-kanMX6

Plasmid

#105148

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu, David Kovar

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3GFP-kanMX6

Use

Tags

3GFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2197

Plasmid

#67551

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306GPD-ccdB-EGFP

Plasmid

#14188

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL08

Plasmid

#104432

Purpose

pHuji in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

pHuji

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

DHFR F[1,2]

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLUC

Plasmid

#1280

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

CUP1-lacZ fusion (CUP1-1 Budding Yeast)

Use

Tags

CUP1 promoter

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
ZJOM141

Plasmid

#133669

Purpose

Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-COX9-mTagBFP2 (COX9 Budding Yeast)

Use

Tags

mTagBFP2

Expression

Yeast

Mutation

Promoter

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-ADH1-CDS1-3HA

Plasmid

#198596

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-ADH1-PAH1-3HA

Plasmid

#198597

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge