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Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only
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PGAL
Plasmid#60337PurposeContains full length native galactose promoterDepositorInsertspromoter
yECitrine
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS314-T7-GFP
Plasmid#33130DepositorInsertGFP with TDH3 5' and 3' UTR (gfp )
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGR420
Plasmid#213782PurposeIntegrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flankDepositorInsertMET15
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT302
Plasmid#41845DepositorInsert4-4-20
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-KanMX4-HO
Plasmid#51662Purposeyeast plasmid for integration of target sequence at HO locus, Kan selectionDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-HDEL
Plasmid#129098PurposeFor labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDELDepositorInsertTEF1p-SS-3xGlyAla-GFP-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Myc-kanMX6
Plasmid#20769DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWKS9
Plasmid#139416PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
UseTagsExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2191
Plasmid#67538PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1
Plasmid#140591PurposeExpress MCART1 in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
UseTagsExpressionYeastMutationcodon-optimized for expression in S. cerevisiaePromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL07
Plasmid#104431Purposemitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertmito-Super Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only