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Plasmid#160382PurposeOverxpression of SKI1/XRN1 in yeastDepositorInsertSKI1
UseTagsExpressionYeastMutationPromoterGALAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA183
Plasmid#74247PurposepSTL1-dPSTRr(2,1)DepositorInsertpSTL1-dPSTRr(2,1)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIYC08
Plasmid#64743PurposeYeast expression plasmid: acsL641P, ALD6, ADH2, ERG10DepositorInsertADH2, ALD6, acsL641P, ERG10
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046348
Plasmid#149108PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046310
Plasmid#149022PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046423
Plasmid#148998PurposeYeast Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKT0149
Plasmid#8740DepositorInsertyESapphire
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with S72A, Y145F, T203IPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymVenus-URA3
Plasmid#168058PurposeReplaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSM1960
Plasmid#41842DepositorInsertSEC63-mRFP
UseTagsmRFPExpressionYeastMutationPromoterAvailable sinceJan. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD
Plasmid#183940PurposeControl vector that has a GPD promoter with no insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-GS
Plasmid#188940PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertpBS-SKII-URA-GAL1-HALO-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK004
Plasmid#171276PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPgpdA ANIA_08041 promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE1c
Plasmid#17461Purposemodified pENTR vector for C-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-GW
Plasmid#63780Purposeyeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insertDepositorTypeEmpty backboneUseTags3xFLAGExpressionYeastMutationPromoterGPDAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
UseTagsGFPExpressionBacterial and YeastMutationPromoterPRC1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only