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Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
UseTagsExpressionBacterial and YeastMutationPromoterREV1Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179060PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomesDepositorInsertRPL18Bp-PIL1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK01
Plasmid#64744PurposeYeast expression plasmid: phaA, phaB, phaCDepositorInsertphaA, phaB, phaC
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
M3926 leu2::KanMX3 Disruptor Converter
Plasmid#51680PurposeYeast marker swap plasmid for converting LEU2 to KanMX3DepositorInsertKanMX3
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
PRS303-Gal1pr-Mag1-sctetR
Plasmid#44753DepositorInsertMAG1-sctetR
UseTagsExpressionYeastMutationPromoterGal1prAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDBD-M
Plasmid#50572PurposeDouble Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571DepositorInsertDHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
UseTagsExpressionYeastMutationPromoterGal1Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBJ1806 pRS413-COF1RFP
Plasmid#37101DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-kanMX6
Plasmid#49176Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneUseTags12PkExpressionYeastMutationPromoterAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx2
Plasmid#104471PurposeIntegrating plasmid for overexpression of OM45-FKBPx2.DepositorInsertOM45p (OM45 Budding Yeast)
UseTagsFKBPx2- HAExpressionYeastMutationPromoterTPI1Available sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-SpHis5
Plasmid#44849PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP255
Plasmid#78934PurposePromoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0413
Plasmid#133470PurposeIntegration into fission yeast genome at his5 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0357
Plasmid#133469PurposeIntegration into fission yeast genome at lys3 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU-mRuby2
Plasmid#176549PurposeExpression of mRuby2 for auxotrophic selection in the absence of uracilDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mRuby2
Plasmid#176547PurposeExpression of mRuby2 for auxotrophic selection in the absence of histidineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1093:SpRY
Plasmid#172188PurposeGenome editing of C. albicansDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only