7,977 results

1. pETconNK-TEM1(S70A,D179G)

   Plasmid

   #81166

   Purpose

   pETconNK with TEM1(S70A,D179G) beta-lactamase

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   GAL1 and gal1

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-SKII-3XHA-HALO-Kanamycin

   Plasmid

   #188933

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Kanamycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIPlac204TKC-GFP-HDEL

   Plasmid

   #21771

   Depositor

   Benjamin Glick

   Article

   Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

   Insert

   Green Fluorescent Protein (GFP)
   

   Use

   Tags

   HDEL and preKar2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS402-nicastrin/APH-1a-HA

   Plasmid

   #207804

   Purpose

   Yeast integrative vector for expression of Nicastrin and APH-1a-HA

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Gamma-secretase subunits nicastrin and Aph1a
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZc-HsACTG1*-thymosinB-8His

   Plasmid

   #111147

   Purpose

   Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   actin gamma 1 (ACTG1 Human)
   

   Use

   Pichia pastoris integration vector

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   codon-optimised for expression in Pichia pastoris

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG426GAL-ccdB-DsRed

   Plasmid

   #14371

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ-pre-pro-alphaf(I)-E2-Crimson

   Plasmid

   #117660

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pABY-c46

   Plasmid

   #201774

   Purpose

   Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046233

   Plasmid

   #149032

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUG-amdSYM-mak10TR

    Plasmid

    #75433

    Purpose

    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

    Depositor

    John McCusker

    Inserts

    MAK10TR
    ApR
    amdSYM
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal 740 bp of MAK10 ORF

    Promoter

    Native promoter and PTEF

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT0209

    Plasmid

    #8730

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEGFP
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGFP-ATG13(315)

    Plasmid

    #122072

    Purpose

    Expressing GFP-Atg13

    Depositor

    Daniel Klionsky

    Article

    Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

    Insert

    ATG13
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JME4390

    Plasmid

    #129656

    Purpose

    LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid

    #165976

    Purpose

    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pphlO6), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPS2351

    Plasmid

    #1352

    Depositor

    Pamela Silver

    Article

    Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

    Insert

    MGA2 (MGA2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pAG425GPD-ccdB-DsRed

    Plasmid

    #14370

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGI3EM09

    Plasmid

    #135482

    Purpose

    Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunHsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p413TEF-Sir2-H364Y

    Plasmid

    #51743

    Purpose

    overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H364Y

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICE URA3

    Plasmid

    #122882

    Purpose

    URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

    Plasmid

    #173456

    Purpose

    Contains mScarletH-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-H-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge