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Plasmid#177103PurposeExpresses HaloTag-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd39T
Plasmid#98303PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.DepositorInsertpdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV104
Plasmid#63194PurposepRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(A503V)
Plasmid#1152DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationA503VPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Kan
Plasmid#188918PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P3
Plasmid#125036PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertENO1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-GFP
Plasmid#166836PurposeExpresses Vac8-GFP in yeasts.DepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-EGFP-ccdB
Plasmid#14322DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-HaloTagx2
Plasmid#177107PurposeExpresses Nhx1-HaloTagx2 in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 1-F GW
Plasmid#58550PurposeMulticopy with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P81nmt1-3FLAG
Plasmid#19347DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-Kan
Plasmid#44956PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selectionDepositorInsertyomRuby
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGal-BE3
Plasmid#172409PurposeYeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoterDepositorInsertBE3
UseCRISPRTagsExpressionYeastMutationPromoterGALLAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only