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Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pML104-HygMx4
Plasmid#83475PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.DepositorInsertHphMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS405-PTEF-Dcr1
Plasmid#22314DepositorInsertDCR1
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS404-PTEF-Ago1
Plasmid#22313DepositorInsertAGO1
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-hpSC_URA3
Plasmid#22317DepositorInsertURA hairpin
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIU211
Plasmid#44036DepositorInsertAGA1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
UseTagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarlet1.4-T2A-mTurquoise2
Plasmid#219849Purposeequimolar expression of mScarlet1.4 and mTurquoise2 in budding yeastDepositorInsertmScarlet1.4-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pML107-kanR468
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 jellyfish, Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pETcon(-)
Plasmid#41522DepositorTypeEmpty backboneUseTags3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…ExpressionYeastMutationPromoterGAL1Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-Cerulean-ccdB
Plasmid#14420DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSPObooster
Plasmid#216160PurposePlasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strainsDepositorUseTagsExpressionYeastMutationMKT1 gene containing an aspartic acid to glycine …Promoterendogenous promoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only