7,943 results

1. pDN0604 (pDEST, HygR)

   Plasmid

   #210483

   Purpose

   pDEST vector with HygR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDN0603 (pDEST, NatR)

   Plasmid

   #210482

   Purpose

   pDEST vector with NatR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCu-MCP-2yeGFP(405)

   Plasmid

   #121419

   Purpose

   Expressing MCP-2yeGFP

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   CUP1p-MCP-2yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMTS-hFluc

   Plasmid

   #177716

   Purpose

   Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   Mitochondrial Targeting Sequence MTS SS

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMTU-DO-URA-C4

   Plasmid

   #160339

   Purpose

   High-copy expression vector for K. marxianus, uracil selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p416 46Q GPD

   Plasmid

   #1178

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pScDD2_ERdd

   Plasmid

   #109047

   Purpose

   destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

   Depositor

   Thomas Wandless

   Insert

   ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
   

   Use

   Tags

   yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

   Expression

   Yeast

   Mutation

   3 mutations : E380G, R434G, N532S

   Promoter

   SOD1

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMJ106: pRS426-His-MBP-NlovFz2_Native

   Plasmid

   #205253

   Purpose

   S. cerevisiae expression vector for NlovFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   NlovFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416-PHO5-GFP-Osh1

   Plasmid

   #58835

   Purpose

   Expresses GFP-Tagged Osh1 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Osh1 (SWH1, OSH1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAG426GAL-EGFP-ccdB

    Plasmid

    #14323

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pI3-MTU-DO-G418

    Plasmid

    #160023

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCUPI NPD-GFP

    Plasmid

    #1191

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    N-terminus of Sup35 (SUP35 Budding Yeast)
    

    Use

    Cup1 promoter

    Tags

    GFP

    Expression

    Yeast

    Mutation

    fused N-terminal Sup35 (~1-253aa) to GFP

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. YIplac211-Sec31-yoHalo

    Plasmid

    #115423

    Purpose

    Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec31-yoHalo
    

    Use

    Tags

    yoHaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-mTID-HA3-KanMX6

    Plasmid

    #227982

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pE5c

    Plasmid

    #17465

    Purpose

    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    ECFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHS2

    Plasmid

    #81037

    Purpose

    HO endonuclease expression

    Depositor

    John McCusker

    Insert

    HYGMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS3867

    Plasmid

    #185744

    Purpose

    Sub-array backbone 3/4

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ZJOM10

    Plasmid

    #133639

    Purpose

    Integration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-Vps4-GFP (VPS4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMJ45: pRS426-His-MBP-SpuFz1_Native

    Plasmid

    #205252

    Purpose

    S. cerevisiae expression vector for SpuFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3413 pBF339 Bcl-XL

    Plasmid

    #8773

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only