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Plasmid#125061PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN6
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KN
Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM291-tetR'-tup11Δ70
Plasmid#41028DepositorInserttetR'-tup11Δ70
UseTagsExpressionYeastMutationtup11 starts at aa70PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P41nmt1-3FLAG
Plasmid#19346DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-DsRed
Plasmid#14364DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSSec7-mGFPx3
Plasmid#25449DepositorInsertSec7 (SEC7 Budding Yeast)
UseTagsmGFPx3ExpressionYeastMutationGFP contains A206K for monomerization.PromoterAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNXgate32-3HA
Plasmid#105076PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneUseTags3xHA and NubGExpressionYeastMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-Kan
Plasmid#44902PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selectionDepositorInsertyomKeima
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LT1_34_sfpHluorin
Plasmid#177707PurposePlasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)DepositorInsertpTEF1-sfpHluorin
UseUsed as donor in genome integration after lineari…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRR2
Plasmid#166097PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRTagsExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP5H
Plasmid#188061PurposePROSS-designed versatile peroxidase 5HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation43 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only