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Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-Kan
Plasmid#44944PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pABY-c15
Plasmid#201768PurposeExpresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterADH1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG305GPD-ccdB
Plasmid#14138DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYZ959
Plasmid#192603PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterScTEF1Available sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAG306GAL-ccdB
Plasmid#14139DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXNubWtgate
Plasmid#105080PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneUseTagsNubIExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP(iL)ExpressionYeastMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS
Plasmid#29591DepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-pphlO6-crtYBI
Plasmid#165977PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICXNHG3
Plasmid#49021PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterAOX1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet26
Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet26 (met26 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet16 (met16 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only