7,935 results

1. gRNA-leu-HYB

   Plasmid

   #64332

   Purpose

   leu2 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of leu2 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pKmK.T4

   Plasmid

   #125056

   Purpose

   Storage plasmid for K.marxianus terminator; type 4 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   PDC1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pESC-URA-GFP-Ubc9ts

   Plasmid

   #20369

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y68L This mutation renders Ubc9 temperature-sens…

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pMTU-DO-G418-C4

   Plasmid

   #160342

   Purpose

   High-copy expression vector for K. marxianus, G418 selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJO672 (pRS415 Gal1-Ufd4-flag)

   Plasmid

   #159590

   Purpose

   expresses Ufd4-flag in yeast cells

   Depositor

   Alexander Varshavsky

   Insert

   Ufd4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDUP3

   Plasmid

   #92174

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS288

   Plasmid

   #200714

   Purpose

   To express yeast Hxt1 hexose transporter in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Inserts

   PMA1 promoter (PMA1 Budding Yeast)
   Hxt1 (HXT1 Budding Yeast)
   ADH2 Ter (ADH2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415GPD-EGFP-ccdB

   Plasmid

   #14314

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNB2289

    Plasmid

    #113396

    Purpose

    Galactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiae

    Depositor

    Suzanne Sandmeyer

    Insert

    Red fluorescent protein, codon optimized for S. cerevisiae
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJRoC30_Th3

    Plasmid

    #188064

    Purpose

    PROSS-designed high-redox potential laccase from Trametes hirsuta

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 mutations, described in publication

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRoC30_Tv9nL

    Plasmid

    #188069

    Purpose

    PROSS-designed high-redox potential laccase from Trametes versicolor

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes versicolor
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    79 PROSS mutations, described in publication

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-G9-5FLAG-hphMX6

    Plasmid

    #49185

    Purpose

    genomic G9-5FLAG epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    G9-5FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP244

    Plasmid

    #78978

    Purpose

    Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179068

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PDR16-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

    Plasmid

    #179063

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-YPT7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG414GPD-ccdB

    Plasmid

    #14144

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3410 pBM272 Bcl-XL

    Plasmid

    #8772

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only