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Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only
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pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAS9c
Plasmid#141250PurposeConstitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-URA
Plasmid#160015PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-LEU
Plasmid#160013PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2225
Plasmid#67553PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pANCH2-Nat
Plasmid#87249Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)DepositorInsertNat
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx1(yeast)
Plasmid#65004PurposeYeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1DepositorInsertGlutaredoxin-1
UseTagsroGFP2ExpressionYeastMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP-Kan
Plasmid#44898PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selectionDepositorInsertyomTagBFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJZC638
Plasmid#62283PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationNuclease activity has been deactivated by mutatio…PromoterpAdh and pGal10Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDM291-tetR-tup11Δ70
Plasmid#41027DepositorInserttetR-tup11Δ70
UseTagsExpressionYeastMutationtup11 starts at aa70PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM100
Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-GA50
Plasmid#84903Purposeinduce expression of dipeptide repeat GA in yeastDepositorInsertGA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC7-mCherry2Bx6
Plasmid#105267PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.DepositorInsertSec7 portion (SEC7 Budding Yeast)
UseTagsmCherry2Bx6ExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only