-
Plasmid#63650PurposepCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB2289
Plasmid#113396PurposeGalactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiaeDepositorInsertRed fluorescent protein, codon optimized for S. cerevisiae
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAS9i
Plasmid#141249PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
Plasmid#24378DepositorInsertpADH1 yeCherry::p150yeGFP-DHFR
UseTagsExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGTy1mhis3-AI or pGTy1his3-AI
Plasmid#62228PurposeA Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.DepositorInsertTy1H3 retrotransposon
UseTagsExpressionYeastMutationPromoterGAL1Available sinceFeb. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG303-Gal-GA50
Plasmid#84907Purposeinduce expression of dipeptide repeat GA in yeastDepositorInsertGA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2792
Plasmid#63653PurposepCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-HPH
Plasmid#160216PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB
Plasmid#14152DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS1209
Plasmid#177340PurposeExpresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3DepositorInsertAequorin
UseTagsExpressionYeastMutationPromoterACT1Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG710_lexO-HO_LexA-TF_HIS3MX
Plasmid#154819PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM18
Plasmid#133631PurposeIntegration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.DepositorInsertp1k-NAB2-GFP (NAB2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only