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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Use

Tags

mEGFP

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRG201

Plasmid

#64394

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203658

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP-Kan

Plasmid

#44898

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
ZJOM43

Plasmid

#133652

Purpose

Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-VPS4-DuDre (VPS4 Budding Yeast)

Use

Tags

DuDre

Expression

Yeast

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGal4AD-CIB1

Plasmid

#28245

Purpose

Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

Gal4AD (CIB1, GAL4 Budding Yeast)

Use

Tags

CIB1

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-FLAG-kanMX6

Plasmid

#20751

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pRS306-PHIS3-GFP-μNS

Plasmid

#116935

Purpose

Yeast integrative vector for the expression of μNS

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

μNS

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

HIS3

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

5S rRNA-tRNA(Arg)

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-Kan

Plasmid

#44956

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTIN7m

Plasmid

#162886

Purpose

Overexpression vector for the fission yeast Schizosaccharomyces pombe (lower strength)

Depositor

Stewart Shuman

Article

Garg et al RNA. 2020 Nov;26(11):1743-1752. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-M-GFP

Plasmid

#11952

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-M-GFP

Use

Tags

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TetO7-HSP30UTR-nLucPEST-MS2(v4)

Plasmid

#199764

Purpose

Control construct of elongation reporter to quantify elongation of LacZ

Depositor

Brian Zid

Article

Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

Insert

NanolucPEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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