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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
EMC1-2EGFP-ter-NAT

Plasmid

#184763

Purpose

Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

EMC1 (EMC1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
ABF2-2EGFP-ter-URA3

Plasmid

#184768

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ABF2 (ABF2 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2Katushka

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

Plasmid

#184774

Purpose

Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL-Aur1-2GFP-Hygromycin

Plasmid

#184775

Purpose

Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proGAL-Aur1 (AUR1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.027

Plasmid

#184964

Purpose

Express -Eco1 RT and ncRNA in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.037

Plasmid

#184966

Purpose

Express -Eco1 dead RT and ncRNA in yeasst

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT

Use

Tags

Expression

Yeast

Mutation

Human codon optimized RT, YXDD catalytic region m…

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge