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Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertpLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymScarletI
Plasmid#193960PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeastDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterial and YeastMutationPromoter2xLexop-minimalCyc1 and PACT1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17
Plasmid#193319PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTagsIAA17ExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT Compatible pAIDA2(6FLAG)
Plasmid#193321PurposeC-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.DepositorInsertTAP-AID*-6FLAG
UseTagsTAP-AID*-6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6MYCExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2Katushka-ter-Hygromycin
Plasmid#184772PurposeRed marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2KatushkaExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6FLAG osTIR1
Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182516PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3HA
Plasmid#182517PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3HAExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3MYC
Plasmid#182518PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIR4 gRNA HIS
Plasmid#182520PurposeExpression vector for gRNA directed against SIR4 ORF.DepositorInsertSIR4 gRNA (SIR4 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAN1 gRNA HIS
Plasmid#182521PurposeExpression vector for gRNA directed against CAN1 ORF.DepositorInsertCAN1 gRNA (CAN1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182522PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT SMASh
Plasmid#182523PurposeC-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182524PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182525PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP DAmP
Plasmid#182511PurposeC-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only