C-SWAT IAA17-6FLAG
(Plasmid
#182522)
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PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMaM482
- Total vector size (bp) 9850
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Vector typeYeast Expression
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Selectable markersMET/CYS
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIAA17
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Tag
/ Fusion Protein
- 6FLAG
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C-SWAT IAA17-6FLAG was a gift from Rob Mitra (Addgene plasmid # 182522 ; http://n2t.net/addgene:182522 ; RRID:Addgene_182522) -
For your References section:
A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast. Shively CA, Dong F, Mitra RD. ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547. 10.1021/acssynbio.2c00547 PubMed 36650116