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Plasmid#215501PurposeA pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1DepositorInsertmECitrine-CtNbr1
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB4887
Plasmid#215502PurposeA pUra4 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB4890
Plasmid#215503PurposeA pAde6 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB4893
Plasmid#215504PurposeA pLys3 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB4896
Plasmid#215505PurposeA pHis5 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
IL1_1
Plasmid#207623PurposeLow copy plasmid expressing mTurquoise2 under the TDH3p promoterDepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterScTDH3Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD101
Plasmid#212925PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-HFL1-8flag-LEU
Plasmid#207035PurposeExpression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertHFL1
UseTags8xFLAGExpressionYeastMutationPromoterpHFL1Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15
Plasmid#207050PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationP85L P86LPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15
Plasmid#207051PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationT56A R65A T87APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15
Plasmid#207052PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationR65A P85A P86APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15
Plasmid#207053PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationR65A P85L P86LPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15
Plasmid#207054PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationL55W T56A V61W R65APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8-MET15
Plasmid#207055PurposeExpression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationPromoterpATG8Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-V5-APEX2-Ura
Plasmid#207061PurposeExpressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.DepositorInsertCox4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-GFP-APEX2-Ura
Plasmid#207062PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pERG6-Pex3-4V5-APEX2-URA
Plasmid#207068PurposeModerate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pLSP1-Pex3-4V5-APEX2-URA
Plasmid#207069PurposeModerate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpLSP1Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_scFv
Plasmid#183733PurposeExpresses anti-lysozyme scFv antibodyDepositorInsertAnti-lysozyme scFv
UseTagsHis6-tagExpressionYeastMutationPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only