Showing: 4021 - 4040 of 7097 results
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Plasmid#160029PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only
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pI6-MTU-DO-URA
Plasmid#160030PurposeIntegrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA13
Plasmid#160127PurposeTo express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-ADE2- HDV-TAMO
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA50
Plasmid#160128PurposeTo express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-LSC2- HDV-TAMO
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-LEU
Plasmid#160176PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-HPH
Plasmid#160207PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pML104-kanR1136
Plasmid#162040PurposeExpresses Cas9 and contains kanR1136 guide which targets the kanR geneDepositorInsertkanR1136 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pML107-kanR468
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaURA3
Plasmid#162461PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaHIS1
Plasmid#162462PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailabilityAcademic Institutions and Nonprofits only -
pG1
Plasmid#162602PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pG2
Plasmid#162603PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 4021 - 4040 of 7097 results