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Plasmid#227606PurposeInducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationR247A,R347APromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1 (SB251)
Plasmid#227600PurposeInducible expression of Strep-tagged Opa1 in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorInsertMFN2 (MFN2 Human)
UseTagsTwin-StrepTag, 10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1(MGD) (SB252)
Plasmid#227601PurposeInducible expression of Strep-tagged Opa1(MGD) in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-580 (MGD) and aa 938-960PromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13247
Plasmid#219918PurposeThe base plasmid of TUNEYALI for TF57DepositorInsertContains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13228
Plasmid#219903PurposeThe base plasmid of TUNEYALI for TF38DepositorInsertContains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13210
Plasmid#219885PurposeThe base plasmid of TUNEYALI for TF20DepositorInsertContains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-KanMX6
Plasmid#228024PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-KanMX6
Plasmid#228026PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-KanMX6
Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BASU-HA3-KanMX6
Plasmid#227978PurposeTo insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertBASU
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BioID2-HA3-KanMX6
Plasmid#227976PurposeTo insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertBioID2
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BirA-HA3-KanMX6
Plasmid#227974PurposeTo insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 markerDepositorInsertBirA
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mGFP in pRS315
Plasmid#221158PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmGFPExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3375_SF_Spt6p-tSH2-1223-1451_NLS
Plasmid#227084PurposeGal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleusDepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsSTREPii-FLAG and SV40 NLS 1ExpressionYeastMutationPromoterGal1-10Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI in pRS315
Plasmid#221159PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI
Plasmid#221157PurposeFluorescent reporter for cytoplasmic aggregatesDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mNeonGreen
Plasmid#221156PurposeFluorescent reporter for cytoplasmic aggregatesDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmNeonGreenExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mNeonGreen in pRS315
Plasmid#221160PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmNeonGreenExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only