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pKmK.T1

Plasmid

#125053

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

INU1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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p403-BrdU-Inc

Plasmid

#71789

Purpose

BrdU-incorporating plasmid with selectable marker HIS3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

Plasmid

#58435

Purpose

Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Use

Tags

Expression

Yeast

Mutation

Promoter

insul-(lexA-box)8-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Km

Plasmid

#46819

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB8644

Plasmid

#126919

Purpose

Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Use

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Mutation

Promoter

TEF

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKmK.I3R

Plasmid

#125065

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1R

Plasmid

#125063

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to LAC4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1L

Plasmid

#125030

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to LAC4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I3L

Plasmid

#125032

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC588

Plasmid

#62315

Purpose

sgRNA with 2x MS2 (wt+f6) for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 (wt+f6) binding module

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-mCherry-SpHis5

Plasmid

#44643

Purpose

Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection

Depositor

Kurt Thorn

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p406GAL1

Plasmid

#17437

Depositor

Fred Cross, Nicolas Buchler

Insert

GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

GAL1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pWS175

Plasmid

#90962

Purpose

Cas9 gap repair vector - HygR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRS316-4M5.3

Plasmid

#45831

Depositor

Dane Wittrup

Insert

4M5.3

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-ccdB-yeastSMASh-YFP

Plasmid

#68854

Purpose

Expresses SMASh tag fused to YFP at N terminus in yeast

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

yeastSMASh-YFP

Use

Tags

SMASh tag

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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