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Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-YFP-yeastSMASh
Plasmid#68855PurposeExpresses SMASh tag fused to YFP at C terminus in yeastDepositorInsertYFP-yeastSMASh
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH2972
Plasmid#100956PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastMutationPromoterpTPGI (galactose+aTc inducible)Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p403-BrdU-Inc
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T1
Plasmid#125053PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertINU1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1
Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)8-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mCherry-SpHis5
Plasmid#44643PurposeYeast tagging vector to create gene fusion with mCherry with SpHis5 selectionDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3R
Plasmid#125065PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1R
Plasmid#125063PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only