8,173 results

1. pROS10

   Plasmid

   #107924

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2224

   Plasmid

   #67547

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2194

   Plasmid

   #67545

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS305-Leu2-PINO4-PfV-GS-Sapphire

   Plasmid

   #116930

   Purpose

   Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Tags

   Sapphire

   Expression

   Yeast

   Promoter

   INO4

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-6xGLY-FLAG-kanMX6

   Plasmid

   #20751

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYDN

   Plasmid

   #162450

   Purpose

   Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

   Expression

   Yeast

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP3AA5

   Plasmid

   #185875

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GPD-ccdB

   Plasmid

   #14148

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGJJ045

   Plasmid

   #183621

   Purpose

   abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

   Depositor

   Ben Lehner

   Article

   Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXP842

    Plasmid

    #46068

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ADH2

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS2

    Plasmid

    #81037

    Purpose

    HO endonuclease expression

    Depositor

    John McCusker

    Insert

    HYGMX
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWS175

    Plasmid

    #90962

    Purpose

    Cas9 gap repair vector - HygR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Tags

    ymScarletI

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIGAhRC

    Plasmid

    #112510

    Purpose

    human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    HIF1B, AhR (ARNT, AHR Human, Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Gal1,10

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTU-DO-HIS-C4

    Plasmid

    #160340

    Purpose

    High-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGal4AD-CIB1

    Plasmid

    #28245

    Purpose

    Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    Gal4AD (CIB1, GAL4 Budding Yeast)
    

    Tags

    CIB1

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK198 (GAL-OsTIR1)

    Plasmid

    #140655

    Purpose

    GAL-OsTIR1(WT)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT)
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTU-DO-LEU-C4

    Plasmid

    #160341

    Purpose

    High-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKT0127

    Plasmid

    #8728

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEGFP
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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