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pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pAG303GAL-ccdB-DsRed

Plasmid

#14349

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pAG306GAL-ccdB-Cerulean

Plasmid

#14379

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Use

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE HDEL

Plasmid

#32118

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Promoter

Available since

Oct. 12, 2011

Availability

Academic Institutions and Nonprofits only
p414GPD_Hsp82_CycTER

Plasmid

#61710

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GPD

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAG303GPD-ccdB-ECFP

Plasmid

#14158

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCGB42/Kan

Plasmid

#17261

Depositor

Stuart Schreiber

Article

Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

Type

Empty backbone

Use

Tags

B42•HA

Expression

Yeast

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pRB306

Plasmid

#8666

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pAG306GPD-ECFP-ccdB

Plasmid

#14284

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG303GAL-ccdB-TAP

Plasmid

#14253

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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p416 3-F GW

Plasmid

#58552

Purpose

pRS416 carrying Gateway cassette for three inserts

Depositor

Leonid Kruglyak

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAG306GPD-Cerulean-ccdB

Plasmid

#14404

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-gib-FFA

Plasmid

#20043

Depositor

Harmit Malik

Article

Elde et al Nature. 2008 Nov 30. ():.

Insert

Protein kinase R

Use

Tags

Expression

Yeast

Mutation

Y489F

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

ADE2

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Use

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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