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pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTG005

Plasmid

#71386

Purpose

localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

RMP1 (RMP1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

ACT1

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCB116

Plasmid

#53365

Purpose

To express Cup9p‐FLAG from the PGAL1 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

Cup9 (CUP9 Budding Yeast)

Use

Tags

Flag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pXP711

Plasmid

#46053

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP712

Plasmid

#46054

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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L1-YA

Plasmid

#15525

Depositor

Vance Lemmon

Article

Cheng et al J Neurochem. 2005 Aug . 94(4):1102-10.

Insert

L1-cytoplasmic domain-Y1176A (L1CAM Human)

Use

Tags

Expression

Yeast

Mutation

Tyrosine 1176 changed to Alanine

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT52

Plasmid

#18843

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT52 (YPT52 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pRS413 Prp5 WT

Plasmid

#87599

Purpose

Encodes Prp5 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 )

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRB2963

Plasmid

#8678

Depositor

David Botstein

Article

Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

Insert

GFP::TUB1-828 under GAL1 promoter (TUB1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pNB789

Plasmid

#60166

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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sup35-C653R

Plasmid

#1198

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed cys 653 to arg

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
Ube2q2

Plasmid

#31425

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2Q2 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pXP312

Plasmid

#26834

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pGBT9-hSyntenin2b

Plasmid

#89422

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

* see below

Promoter

ADH1

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT1

Plasmid

#18848

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT1 (YPT1 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pLUK

Plasmid

#64184

Purpose

harbours marker genes LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNXRVat-HACre

Plasmid

#78399

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
TKanAX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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