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Plasmid#1197DepositorInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDR-AmTrac-LS
Plasmid#47767Purposeexpresses AmTrac-LS in yeastDepositorInsertAmTrac-LS
UseTagsmcpGFPExpressionYeastMutationPromoterPMAAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2HGAL/CGR/CYC
Plasmid#1218DepositorInsertCGR (Nr3c1 Rat)
UseTagsExpressionYeastMutationpRS423 with GAL promoter and CYC terminator gene…PromoterAvailable sinceJuly 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-NYFP
Plasmid#99557PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB-DsRed
Plasmid#14368DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_AH
Plasmid#98556Purposeempty BB3 for insertion and overexpression of 7 genes in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
M34 Xdsh PDZ bait-pGBT9
Plasmid#17190DepositorInsertXdsh (dvl2.L Frog)
UseTagsGal4-DBDExpressionYeastMutationDsh bp 343-1191PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHK
Plasmid#64187Purposeharbours marker genes HIS3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
Plasmid#50033PurposeGalactose-inducible expression of ER-membrane S. pyogenes SrtA in yeastDepositorInsertSrtA(strep)
UseTagsHAExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Lys13Arg
Plasmid#50722Purposeexpression of the human TPI Lys13Arg allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationchanged Lysine 13 to ArgeninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Thr
Plasmid#50721Purposeexpression of the human TPI Ile170Thr allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ThreoninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB4780
Plasmid#106143PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pWHI2-NAT
Plasmid#80588PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterNative WHI2 promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH743-CEN-RLuc/slowstaCFLuc
Plasmid#38223DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only