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Plasmid#78968PurposePromoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL-CDC12(WT)
Plasmid#1165DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
GAL-CDC12(K351N)
Plasmid#1166DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsExpressionYeastMutationK351NPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
CDC12(E368Q)
Plasmid#1167DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTags266bp of upstream sequenceExpressionYeastMutationE368QPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pJW3.42
Plasmid#62866PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusionDepositorInsertPPTR-1/pLexA-N bait
UseTwo-hybrid bait vectorTagsLexAExpressionYeastMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K740R
Plasmid#111953PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutationDepositorInsertHsh155 K740R
UseTagsExpressionBacterial and YeastMutationK740RPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWS936
Plasmid#123050PurposeG_ subunit for creating C-terminal transplantsDepositorInsertGpa1 C-terminal Truncation Vector [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS067
Plasmid#123044Purpose_-factor GPCRDepositorInsertSte2 [3]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS053
Plasmid#123025PurposePheromone responsive promoterDepositorInsertpFIG1 [2]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS060
Plasmid#123024PurposePheromone responsive promoterDepositorInsertpFUS1 [2]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorInsertRAD30 (RAD30 Budding Yeast)
UseTagsc-mycExpressionYeastMutationPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pleum
Plasmid#60333PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS810
Plasmid#8936DepositorTypeEmpty backboneUseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only