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720-2µ-maxHIS3

Plasmid

#40612

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJJH1338

Plasmid

#36912

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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Ube2d3

Plasmid

#31411

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D3 Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pXP832

Plasmid

#46066

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGLx2-SET1

Plasmid

#53256

Purpose

GAL driven LexA-tagged SET1

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Use

Tags

LexA

Expression

Yeast

Mutation

Promoter

GAL1/10

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pXP811

Plasmid

#46061

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pY128

Plasmid

#127713

Purpose

Level 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG425GPD-ccdB-EYFP

Plasmid

#14226

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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AtABCG11

Plasmid

#117091

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

AtABCG11

Use

Tags

FLAG and His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGBT9-mCALEBcyt (aa 447-539)

Plasmid

#89404

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Insert

CSPG5 (Cspg5 Mouse)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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LD223

Plasmid

#69190

Purpose

Integration of 2GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg23-2EGFP

Use

Tags

2xEGFP

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-ccdB-Cerulean

Plasmid

#14386

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-ccdB-TAP

Plasmid

#14262

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pAG304GPD-ccdB-EGFP

Plasmid

#14184

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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Ube2t

Plasmid

#31427

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2T Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDSM-ab-Psptef1-NAT-Ttip1

Plasmid

#127747

Purpose

Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

nourseothricin N-acetyltransferase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Psptef1

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB-EYFP

Plasmid

#14225

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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