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Plasmid#149019PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAS2-1-Esf1
Plasmid#99987PurposeExpress yeast Esf1 with Y2H binding domain fusionDepositorInsertEsf1 (ESF1 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-Cerulean-ccdB
Plasmid#14405DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAH235
Plasmid#121145PurposeCas9 expression controlled by the nmt41 promoterDepositorInserthumanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoternmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-EYFP
Plasmid#14215DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG304GPD-ccdB-HA
Plasmid#14232DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2-T2A-mCherry
Plasmid#118442PurposeProduces equimolar expression of yeast codon-optimized mTq2 and mCherryDepositorInsertymTurquoise2-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-KanMx
Plasmid#122917PurposeADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A10-17
Plasmid#112826PurposepSMF2 with all S to A mutations in only repeats 10-17.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A2-9
Plasmid#112825PurposepSMF2 with all S to A mutations in only repeats 2-9.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 2…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A18-25
Plasmid#112827PurposepSMF2 with all S to A mutations in only repeats 18-25.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 WT
Plasmid#87288PurposeEncodes Y2H Gal4 activation domain for Hsh155 WTDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS423-SgH
Plasmid#163972PurposeHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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FRP322
Plasmid#159303PurposeYeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2del, S3del, F64L, A206K, L231HPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mRuby2-T2A-mTurquoise2
Plasmid#118429PurposeProduces equimolar expression of mRuby2 and mTq2DepositorInsertmRuby2-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-Cerulean-ccdB
Plasmid#14410DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR526
Plasmid#1122DepositorUseTagsGR526ExpressionYeastMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-Venus-FLAG-LINXa4-Bre1
Plasmid#73618PurposeVisualizing the nuclear translocation of LINXa4-Bre1 in yeast.DepositorInsertVenus
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only