Addgene

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pAG425GPD-ccdB-ECFP

Plasmid

#14178

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pJT303_SNR52_sgRNA_Can1-3

Plasmid

#145066

Purpose

Expresses sgRNA targeting Can1-3 site

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

Can1-3 sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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CMYA5/pACT2

Plasmid

#97208

Purpose

CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pUR-sap1+

Plasmid

#15984

Depositor

Eishi Noguchi

Article

Noguchi et al Genetics. 2007 Feb . 175(2):553-66.

Insert

Sap1 (sap1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only
pDRF1-GW tdTomato-T2A-mTurquoise2

Plasmid

#118439

Purpose

Produces equimolar expression of tdTomato and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tdTomato-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CUP Snf5 YFP p316

Plasmid

#15597

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

SNF5 (SNF5 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pR5-30

Plasmid

#22290

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

D681, E682->AA

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pR5-19

Plasmid

#22288

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

C914, C917->AA in the C3HC4 ring-finger motif

Promoter

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only
pJKW1847

Plasmid

#174004

Purpose

Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP94D108
ATR1 (ATR1 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pREP4xN_ccdB

Plasmid

#79022

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

Depositor

Pernilla Bjerling, Göran Akusjärvi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGPDluxAB(Ura)

Plasmid

#1119

Depositor

Susan Lindquist

Article

Singer et al Mol Cell 1998 Apr;1(5):639-48.

Insert

bacterial luciferase

Use

Tags

Expression

Yeast

Mutation

n/a

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
FT1

Plasmid

#31435

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Fused Toes Homolog (AKTIP Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLEP1-H-Hsp82

Plasmid

#1277

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

his-Hsp82 (HSP82 Budding Yeast)

Use

Tags

Histidinex6

Expression

Yeast

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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