Showing: 3061 - 3080 of 7097 results
-
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
-
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationPromoterAOX1AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationR201APromoterAOX1AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
UseTagsExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …AvailabilityAcademic Institutions and Nonprofits only -
pCggRO-reporter
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆266-280)
Plasmid#121520PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 266-280PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆266-312)
Plasmid#121521PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 266-312PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆279-295)
Plasmid#121522PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 279-295PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆296-312)
Plasmid#121523PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 296-312PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆313-334)
Plasmid#121524PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 313-334PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆335-417)
Plasmid#121525PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 335-417*PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LANS1-Set2
Plasmid#122002PurposeFLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeastDepositorInserthistone methyltransferase SET2 (SET2 Budding Yeast)
UseSynthetic BiologyTagsFLAGExpressionYeastMutationSet2 point mutations K538G, K539S, R549G, K550S, …PromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-mVenus-FLAG-LANS1-Set2
Plasmid#122003PurposemVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeastDepositorInsertmVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
UseSynthetic BiologyTagsmVenus-FLAGExpressionYeastMutationSet2 point mutations K538G, K539S, R549G, K550S, …PromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pGAL-GFP kan
Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1 and GAL1 comes in pYES2AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 3061 - 3080 of 7097 results